Homogeneous enzyme immune assay for staphylococcal enterotoxin B.
نویسندگان
چکیده
A simple homogeneous enzyme immune assay was developed for detection of staphylococcal enterotoxin B by a conjugate of beta-amylase coupled with the enterotoxin.
منابع مشابه
جستجوی توکسین‌های استافیلوکوکی (توکسین شوک توکسیک، انتروتوکسین‌های آ، بی، سی، دی) در مایع گوش میانی بیماران مبتلا به اوتیت مدیا همراه با افوزیون: گزارش کوتاه
Background: Staphylococcal superantigens (SAg;aposs) may have some role in otitis media with effusion (OME). The aim of this study was the search of staphylococcal SAg;aposs in middle ear effusion of children with OME. Methods: This cross sectional-analytic study was done in ENT & pediatric wards upon 64 children with otitis media with effusion (OME) between 1-15 years, (mean age=7.42+4 years)...
متن کاملSpecificity and cross-reactivity of staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, C1, D, and E.
The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C1, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude ...
متن کاملNature and reactivity of staphylococcal enterotoxin A monoclonal antibodies.
Monoclonal antibodies from four clones (C5, C3, B2II, and B2I) directed against staphylococcal enterotoxin A were tested by the indirect enzyme-linked immunosorbent assay and double-gel immunodiffusion (micro-Ouchterlony) assay for the nature of heavy and light chain types. The reactivities of monoclonal antibodies were also tested by indirect enzyme-linked immunosorbent assay with various leve...
متن کاملMonoclonal Antibody-Based Sandwich ELISA for the Detection of Staphylococcal Enterotoxin A
A sensitive and specific monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated for the detection of staphylococcal enterotoxin A (SEA). After routine fusion and selection, 10 monoclonal antibodies showed high affinity for SEA. An optimal pair for sandwich ELISA was selected by pairwise interaction analysis. After optimization, the limit of de...
متن کاملRapid quantitative serological assay of staphylococcal enterotoxin B.
A simple, rapid method, based on the Oudin single diffusion technique, is described for the quantitative assay of staphylococcal enterotoxin B. The method yields reproducible results without close control of such assay variables as temperature, antiserum dilution, and assay time, provided that the ionic strength is maintained above 0.2 n sodium chloride equivalent.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Infection and immunity
دوره 21 2 شماره
صفحات -
تاریخ انتشار 1978